??? ? ? ? ? ? ?Hcy?? ? ? ? ? ? ? ? ??[1]????[2]????????????????????[3]??????????[4]??????????Hcy????????????????? ? ? ? ? ? ?? ? ? ? ? ? ? ? ? ? ??MTHFR?????????????MTRR??Hcy?????????????Hcy?????????????????B12??????5-??????????????????
??5-???????MTHFR??5,10-????????????????B12??MTRR????????????????????????????????
??MTHFR?MTRR??????????????? ? ? ? ? ? ?MTHFR C677T?A1298C?MTRR A66G,????????????????????[5]???????????[6]????Hcy??[7].?????????Hcy???????????????????????????????????????????????????????????????????????????????MTHFR?MTRR????????????????????????????????????????????????????????????????
???????
????????
???????2013?4??2015?2?????????????????????????????????????????????????????????????????????DNA??????????????????????????????????????????????????????????????????3843????20~48?27.26±4.15????????????????????????????60%.???????????????????????????????????????????????????????
????????????DNA??
??????????????????????????????DNA.??Taqman-MGB????MTHFR C677T?A1298C?MTRR A66G???????????????????ABI????????????????PCR????????????10μl,?????20ng/μl?DNA??1μl,2×Taqman Universal MasterMix5μl,20×Taqman-MGB??0.5μl,????3.5μl.
???????95? 10min,????20???????92?15s,60?1min?????30???????89?15s,60?90s????????ABI 7900?????PCR??????????????????????????????????
?????????
????SPSS 17.0????????????????????????HaploView 4.2???????????????????????????Hardy-Weinberg????????????????????????????????????????P<0.05??????????
????
????????MTHFR?MTRR?????????????????????
??????????MTHFR C677T??????? ? ? ? ? ? ? ? ? ? ? ?[8]?? ?[9]???[10]???[11]???[12]???[13]???[14]???[15]???[16]???[17]???[18]???[19]???????????P<0.01??
??MTHFR 677TT??????????18.7%,????????????????????????????????????????P<0.01??????4?1A,?2A?????????MTHFR A1298C???????????????????????????????????????????P<0.01??MTHFRA1298C????????????????????????????????????????????P<0.05?P<0.01??????4?1B,?2B?????????MTRR A66G?????????? ???? ???? ? ? ? ? ? ? ??P<0.01??
??MTRR A66G??????????????????????????????P<0.05?P<0.01??????4?1C,?2C?
????MTHFR C677T?A1298C?????????????
??MTHFR C677T?A1298C????????
??CC/AA 635??16.5%??CC/AC 537??14.0%??CC/CC 112??2.9%??CT/AA 1262??32.8%??CT/AC 575??15.0%??CT/CC 2??0.1%??TT/AA 718??18.7%??TT/AC 2??0.1%??TT/CC0????????????????
??CA?40.0%??TA?42.6%??CC?17.4%??TC?0.1%?????????????????D′=0.988,r2=0.154??????????????????[20].
????
??????????????????????????2001~2010???????????????????????????????[21].???????????????????????
??MTHFR?MTRR???????Hcy???????????? ??? ? ? ? ? ? ? ??? ???????????????????MTHFRC677T?A1298C?MTRR A66G????????????????????
??MTHFR???????1p36.3?????19.3kb,mRNA??7105bp,??12?????
??C6777T???5????????????????????????MTHFR?????????????677CC??????TT???????????????22%.????[22]??Meta??????677CC?????????MTHFR677TT?CT?????????????????????OR????3.13?1.66.
??A1298C???8??????????????????????
??MTHFR 677CT?1298AC???????????????????36%[23].MTRR???????5p15.3~p15.2,??32 021bp,?15?????
??A66G???2????????????????????????MTRR 66AA????????66GG???????????????OR=2.6,95%????1.3~5.3?[24].?3???????????????????[25]??MTHFR C677T?A1298C?MTRR A66G???????????????????????????????????????????????????19.45%?31.07%,???????????????????????OR?2.265,?????????????????????????????
??????????????????????MTHFR C677T?A1298C?MTRR A66G????????????????????????????????????????????????????????????????????????????????????????????? ? ? ? ??? ? ? ? ? ??MTHFR C677T?A1298C??????????????????? ???? ?????[20].?????? ?MTHFR C677T?A1298C?MTRR A66G??????????????????????????????????????
??MTHFR 677T????????????????????????????????????????????????????????????????????????????????????????MTHFR?MTRR????????????????????????????????????????????????????????????????????????????????????
????MTHFR?MTRR?????????????????????????????????????????????????????????????MTHFR?MTRR??????????????????????????????????????????????????????????????????????????????????
??? ? ? ?
??[1] ???????????????????????[J].??????????2009,17?5??1-4.
??[2] ????????????????????????????MTHFR??C677T??????[J].??????????2011,19?2??40-42.
??[3] ?????????????????????????????????????????[J].?????????2005,8?3??160-164.
??[4] ?????????????????????????????[J].???????2011,26?24??3822-3824.
??5-???????MTHFR??5,10-????????????????B12??MTRR????????????????????????????????
??MTHFR?MTRR??????????????? ? ? ? ? ? ?MTHFR C677T?A1298C?MTRR A66G,????????????????????[5]???????????[6]????Hcy??[7].?????????Hcy???????????????????????????????????????????????????????????????????????????????MTHFR?MTRR????????????????????????????????????????????????????????????????
???????
????????
???????2013?4??2015?2?????????????????????????????????????????????????????????????????????DNA??????????????????????????????????????????????????????????????????3843????20~48?27.26±4.15????????????????????????????60%.???????????????????????????????????????????????????????
????????????DNA??
??????????????????????????????DNA.??Taqman-MGB????MTHFR C677T?A1298C?MTRR A66G???????????????????ABI????????????????PCR????????????10μl,?????20ng/μl?DNA??1μl,2×Taqman Universal MasterMix5μl,20×Taqman-MGB??0.5μl,????3.5μl.
???????95? 10min,????20???????92?15s,60?1min?????30???????89?15s,60?90s????????ABI 7900?????PCR??????????????????????????????????
?????????
????SPSS 17.0????????????????????????HaploView 4.2???????????????????????????Hardy-Weinberg????????????????????????????????????????P<0.05??????????
????
????????MTHFR?MTRR?????????????????????
??????????MTHFR C677T??????? ? ? ? ? ? ? ? ? ? ? ?[8]?? ?[9]???[10]???[11]???[12]???[13]???[14]???[15]???[16]???[17]???[18]???[19]???????????P<0.01??
??MTHFR 677TT??????????18.7%,????????????????????????????????????????P<0.01??????4?1A,?2A?????????MTHFR A1298C???????????????????????????????????????????P<0.01??MTHFRA1298C????????????????????????????????????????????P<0.05?P<0.01??????4?1B,?2B?????????MTRR A66G?????????? ???? ???? ? ? ? ? ? ? ??P<0.01??
??MTRR A66G??????????????????????????????P<0.05?P<0.01??????4?1C,?2C?
????MTHFR C677T?A1298C?????????????
??MTHFR C677T?A1298C????????
??CC/AA 635??16.5%??CC/AC 537??14.0%??CC/CC 112??2.9%??CT/AA 1262??32.8%??CT/AC 575??15.0%??CT/CC 2??0.1%??TT/AA 718??18.7%??TT/AC 2??0.1%??TT/CC0????????????????
??CA?40.0%??TA?42.6%??CC?17.4%??TC?0.1%?????????????????D′=0.988,r2=0.154??????????????????[20].
????
??????????????????????????2001~2010???????????????????????????????[21].???????????????????????
??MTHFR?MTRR???????Hcy???????????? ??? ? ? ? ? ? ? ??? ???????????????????MTHFRC677T?A1298C?MTRR A66G????????????????????
??MTHFR???????1p36.3?????19.3kb,mRNA??7105bp,??12?????
??C6777T???5????????????????????????MTHFR?????????????677CC??????TT???????????????22%.????[22]??Meta??????677CC?????????MTHFR677TT?CT?????????????????????OR????3.13?1.66.
??A1298C???8??????????????????????
??MTHFR 677CT?1298AC???????????????????36%[23].MTRR???????5p15.3~p15.2,??32 021bp,?15?????
??A66G???2????????????????????????MTRR 66AA????????66GG???????????????OR=2.6,95%????1.3~5.3?[24].?3???????????????????[25]??MTHFR C677T?A1298C?MTRR A66G???????????????????????????????????????????????????19.45%?31.07%,???????????????????????OR?2.265,?????????????????????????????
??????????????????????MTHFR C677T?A1298C?MTRR A66G????????????????????????????????????????????????????????????????????????????????????????????? ? ? ? ??? ? ? ? ? ??MTHFR C677T?A1298C??????????????????? ???? ?????[20].?????? ?MTHFR C677T?A1298C?MTRR A66G??????????????????????????????????????
??MTHFR 677T????????????????????????????????????????????????????????????????????????????????????????MTHFR?MTRR????????????????????????????????????????????????????????????????????????????????????
????MTHFR?MTRR?????????????????????????????????????????????????????????????MTHFR?MTRR??????????????????????????????????????????????????????????????????????????????????
??? ? ? ?
??[1] ???????????????????????[J].??????????2009,17?5??1-4.
??[2] ????????????????????????????MTHFR??C677T??????[J].??????????2011,19?2??40-42.
??[3] ?????????????????????????????????????????[J].?????????2005,8?3??160-164.
??[4] ?????????????????????????????[J].???????2011,26?24??3822-3824.