英语论文
原创论文
留学生作业
英语论文格式
免费论文
essay
英国硕士论文
英国毕业论文
英语论文
留学生论文
澳大利亚论文
新西兰论文
澳洲Report
澳洲留学生论文
美国留学论文
Dissertation
美国硕博论文
essay case
Eassy
Term paper
英语毕业论文
英文论文
课程作业
德语论文
德语专业论文
德语本科论文
德国留学论文
Assignment
日语论文
韩语论文
法语论文
俄语论文

脓毒症早期心肌损伤与炎症因子的关系

时间:2015-10-28 来源:未知 编辑:梦想论文 阅读:

Sepsis is a systemic inflammatory response induced by bacterial infection, which can lead to severe sepsis, septic shock and multiple organ dysfunction syndrome by further development of [1]. Myocardial injury is a common complication of sepsis, the incidence rate is about 50%[2], it is an important reason for the prognosis of sepsis, and is also an important reason for the increase of the mortality of sepsis. Severe sepsis through inflammatory mediators, inflammatory mediators, inflammatory mediators and the interaction of different sources of factors, in order to fall in the effect of myocardial injury. Therefore to explore the links between sepsis early inflammatory factor and the change of myocardial injury, on myocardial protection and sepsis treatment has a great help, we observe the purulent septic rats early serum cardiac troponin I (cardiac troponin cTnI), tumor necrosis factor sub - alpha (alpha tumournecrosis factor TNF alpha in), leukocyte mediated endothelin-1 (IL-1) change to find a link between the pus poisonous disease early myocardial damage and inflammatory factors.

 

1 materials and methods

 

72 experimental animals and group clean grade SD rats 1.1, all female, body weight (190.50 + 20.20) g, purchased from Medical University Of Anhui animal experiment center, randomly divided into sham operation group and sepsis group, 36 rats in each group.

 

1 animal models were purchased for 1.2 weeks after the purchase, and the animals were fed with CLP[3] in accordance with the method of the experiment. Weighing, 10% 3mL (0 /100g) subcutaneous injection of anesthesia, fixed, sterile towel. Around 1.5 cm incision was made along the midline of the abdomen, and the appendix was found in the root of the appendix. With 18 needle puncture of the 3 times of the appendix, to prevent the needle hole closed, the extrusion of the appendix caused by fecal overflow. After the abdominal cavity, the abdominal cavity, a layer by layer suture abdominal incision. At the end of surgery, the animals were intraperitoneally injected with saline 5 mL for prevention of shock.

 

6 specimens were prepared with the method of two groups of animals at 9, 24, 36, 48, 72 and 3 h after 5 blood samples were collected from the abdominal aorta, 6 mL, 4 h, 4000 r/min, 200 min, 2 EP, 15 L, respectively, were placed in the -80, and to avoid the influence of different batches of experimental and reagent.

 

1.4 detection index using double antibody sandwich enzyme immunoassay method to determine the level of cTnI, IL-1, TNF- in two groups of specimens, the operation is strictly in accordance with the requirements of the kit.

 

1.5 statistical methods using SPSS 13 statistical analysis, measurement data with mean + standard deviation (x + s), statistical analysis using t test, correlation analysis using Spearman rank correlation analysis, P < 0.05 for the difference was statistically significant.

 

2 Results

 

Two serum cTnI levels were detected in the 2.1 groups of serum cTnI at 3 h reached peak, followed by a downward trend, and sepsis group were significantly higher than the sham operation group (<0. P 05), 3 h reached a peak, see Table 1

 

2.2 serum TNF- and IL-1 were detected in two groups of serum TNF-, IL-1 in 3 h reached peak, followed by a downward trend, and sepsis group were significantly higher than the sham operation group (<0. P 05), see Table 2, table 3

 

2.3 serum TNF-, IL-1 and TNF- were correlated with cTnI, IL-1 and cTnI were significantly positively correlated with cTnI, and and TNF- were significantly positively correlated (r = 0.393, P = 0.164), cTnI and IL-1 had significantly positive correlation (=0. r 980, =0. P 000).


 
3 discussion

 

The mechanism of myocardial injury in sepsis is not determined, but it may be important for [4]. sepsis combined with myocardial damage. However, the clinical symptoms of myocardial damage caused by bacterial toxins, immune regulation, myocardial energy metabolism disorder, myocardial cell apoptosis and gene regulation are important factors in the development of sepsis. However, the early stage of sepsis is reversible. The myocardial cells are "dormant" [5]. So it is very important to detect early detection index.

 

CTnI in myocardial cell injury occurs when the cell membrane is rapidly released into the blood, the early sensitivity of cTnI, cT-nI in diagnosis of myocardial necrosis and myocardial damage of 93% ~ 100%, specificity of 96% ~ 98%, can determine the minor myocardial damage, which is considered to reflect the degree of myocardial injury in the early [6,7]. of the patients with sepsis, which is also consistent with the report, and the number of patients with sepsis group 7 ~8 times, it also shows that it has a high sensitivity. In recent years, cTnI can reflect the degree of myocardial injury and the prognosis of [8,9].

 

Under the stimulation of TNF-, the activation of monocytes and macrophages produce inflammatory mediators such as IL-1[10] and TNF-, which is one of the central mediators in the development of the disease. IL-1 as a kind of proinflammatory cytokines, can induce II-6, TNF-, and so on. The study shows that IL-1 can be induced by signal transduction and gene regulation. [11]. and [12] in the myocardium of myocardial dysfunction in sepsis rats were higher than those in the control group. In this study, the levels of serum, TNF- and H reached the peak at 3 IL-1, and the levels of serum and were significantly higher than those in the sham operation group. , IL-1 and TNF- in serum were consistent with the increase of cTnI, the correlation analysis showed a high degree of correlation, so the early detection of TNF- and IL-1 in the early stage of sepsis can be used as an important index for the diagnosis of myocardial injury in early sepsis.

 

Reference

 

Cornell TT Wynn, J et, TP Shanley, al. Mechanisms and regu-lation of the gene-expression response to sepsis [J]. Pediat-rics [1], 2010125 (6): 1258 - 1248

 

Vieillard-Baron A Caille, V [2], C Charron, al. Actual inci-dence of global left ventricular hypokinesia in et septicshock[J]. adult Care Crit Med, 2008,36 (6): 1706 - 1701

 

[3] Tang Yaoqing, Li Lei. Animal model of sepsis and its application in the Chinese Journal of experimental surgery, 2006,23 (12): 1433 -1434.

 

[4] Jin Guoqiang, Wu Dan. Progress in the pathogenesis of myocardial injury in sepsis [J]. Journal of Nanchang University (Medical Edition), 2010,50 (12): 119 -122.

 

[5] Chen Xuyan, Li Yingli. The effect of cytokines on heart function in patients with sepsis [J]. Chinese Journal of emergency medicine and disaster medicine, 2007,2 (5): 308 -310.

 

Rudiger A Singer, M. Mechanisms of sepsis-induced cardiacdysfunction[J]. Crit Care Med [6], 2007,35 (6): 1608 - 1599

 

[7] He Weihua, Wang Shichang, Pan Haiqing. Assessment of the short-term prognosis of patients with congestive heart failure ([J].), 2012,33 (1495): 11 - 1493

 

[8] Chen Ming, Dai Hong. CK-MB [J]. in the early diagnosis of acute myocardial infarction CTnI Anhui medicine, 2008,12 (5): 433 -434.

 

[9] Zheng Liang Peng, Lu Yu, Wei Hua Tan, 481.. sepsis patients serum calcitonin (original and cardiac troponin I level correlation [J]. Jilin Medical and 2014,35 (3),

 

[10] Jiang Longyuan, Luo Zhengchao, Zhang Meng. Ulinastatin he Ding of sepsis in rats brain tissue factor alpha and leukocyte mediated factor [J]. The Journal of medicine, 2008,7 (4): 70-74. (12): 1234 1237.

 

[11] Zhuang Haizhou. The mechanism of myocardial injury in patients with sepsis and the mechanism of myocardial injury in patients with sepsis, 2008,24 (24): 4313 -4315.

 

[12] Xiao Min Dong, Fang Lin Sen, Gezhen,. Pus sepsis rats Dao and TNF alpha changes [J]. Anhui medical, 2012,33 (11): 1415 -1417.

分享到:
------分隔线----------------------------
发表评论
请自觉遵守互联网相关的政策法规,严禁发布色情、暴力、反动的言论。
最新评论
随机推荐英语毕业论文