0 Introduction
The collection of blood samples and the separation of serum is very important in the control of clinical biochemical analysis before the [l]. The isolation layer formed by the separating glue can be tightly attached to the wall of the test tube, and can effectively isolate the contact between the serum and the blood cell, thereby ensuring the purity of the serum is reduced by 3. But the current domestic production of separation gel tube quality uneven, the separation gel of poor quality may not be completely separated from the serum and blood cells, and can not completely prevent the red blood cells with high content of substances released into the serum. In this study, we analyzed the detection value of the isolated serum and the isolated serum in the different time of preservation of the same sample.
1 objects and methods
1.1 general information.
1.1.1 data were selected from 30 outpatients in our hospital in July 1, 2014:
1.1.2 separator tube using the separation gel / Guangzhou Yang Pu Medical Technology Co., Ltd. production of vacuum blood coagulant.
1.1.3 Nanjing zhaoshida PSD electrolyte analyzer 15 B electrolyte analyzer.
1.2 experimental methods.
1.2.1 collection: in the consent of patients or their families consent, by separating gel / coagulant vacuum blood collection outpatient fasting venous blood of 30 copies, each sml. per tube to collect immediately after mixing a 4 upside down 5 times, at room temperature for 30 minutes with 3500 rpm speed from the heart 10 minutes to prepare the serum half stored in the original test tube (hereinafter referred to unfractionated serum), half separated to microcentrifuge tube preservation (hereinafter called serum separation).
1.2.2 specimens were detected: 0.5h, 6h, 12h, 30h, and K, NA and CL levels were measured in the serum and the isolated serum respectively after the blood collection, and each specimen was measured 3 times.
1.2.3 specimen preservation: after each test is completed, the separated serum and serum are placed in the refrigerator for 4 degrees.
1.2.4 was examined by s data: cabinet scholar) said, SPSS13.ofo:WindowS software package was used to analyze the single sample t test and ANOVA, p<0.05 difference was statistically significant.
2 Results
Analysis of the results of 30 cases of isolated serum and separated serum at different times in Table 1 results from table 1 showed that there was no significant difference between the 2 methods in 12h. 24h after the determination of the results of the 2 methods of preservation of serum no significant difference, and the results of K and NA increased, the difference was statistically significant (CL) (P<0.05). At the same time, the electrolyte composition was stable after 30 hours, and there was no statistical significance (>0.05) in the 30 hours. When the serum was not isolated, the electrolyte composition was stable in 12 hours, and there was no statistical significance (P>0.05). After 24 hours, the results of K and NA were increased compared with those of 0.5 hours, and the difference was statistically significant (<0.05). The rising trend of K and NA are shown in Figure 1 and Figure 2. It can be seen from Figure 1 and Figure 2 that the rising rate of K and NA in isolated serum is higher than that of isolated serum.
3 discussion
By what we do experiments that the serum separation gel tube and blood cells separated, reduced blood electrolyte release into the serum and the influence of the determination results of electrolyte array 8, but can only save time in 12 hours, saving 24 hours after K, the results of NA changes were statistically significant (probably not completely isolated blood separating gel and serum induced). In our daily work, most of the electrolyte testing can be completed within 4 hours. Therefore, under normal circumstances, the analysis of specimens stored directly in the use of the brand of the separation of the glue tube can meet the requirements of stability analysis. But if special circumstances were not timely detection, we should evaluate the samples need to save time, as can be detected within 12 hours, it can be stored directly in the electrolyte analysis sample separation gel tube, such as 12 hours after the test, then we should be separated from the serum electrolyte microcentrifuge tube preservation, to ensure the stability of electrolyte composition.
At the same time, in order to ensure the separation gel preparation control serum samples of high quality, we should regulate the separation gel coagulant blood using the steps as follows: immediately after blood collection tube 4 gently inverted 5 times to mix specimens. Wait for the specimen to be fully coagulated (30 minutes to be placed) for one minute, 3500~4000r/min centrifugation for 10 minutes, and serum and blood clots are completely separated by gel separation. If the centrifugal speed is low, acting on the separation gel is weak, will lead to poor blood return separation gel; if it is not completely solidified centrifugal, can appear fibrin flocculent retention in serum or colloid layer may stay in the serum protein fiber silk is very easy to plug the needle. Therefore, it is better to have a better centrifugal effect than the routine biochemical examination after the blood is completely solidified.